Product description

This course provides a detailed introduction to the fundamental principles and hands-on techniques of microscopy. Participants will learn about the essential components and configurations of microscopes, including light paths, resolution, and magnification, with a focus on achieving optimal image quality.

• Key topics include an overview of brightfield microscopy and its components, the Köhler illumination setup and alignment process, and an introduction to phase contrast microscopy. Through practical, hands-on sessions, you will gain valuable experience in setting up and using brightfield and phase contrast microscopes effectively.
• By the end of the course, you will have a solid understanding of microscope configurations, be able to apply the Köhler illumination alignment process, and confidently use phase contrast techniques for enhanced image clarity in various biological and scientific applications.
• Learn about microscope components, configurations, resolution, and magnification, including brightfield and phase contrast techniques, with hands-on practice time.
• Learn about microscope components, configurations and light paths
• Learn about resolution and magnification
• Gain an overview of brightfield and its components
• Find out about the Köhler illumination set-up alignment process
• Receive an introduction to the phase contrast technique and phase contrast components
• Benefit from hands-on practice time with the microscope for brightfield and phase contrast.
• The course covers:
• History, Microscope Anatomy, Magnification, Resolution, Diffraction & Köhler (Lecture)
• Köhler (Practical)
• Know your Lens: Aberration, Lens Design, Working Distance, Refractive Index Matching & Nomenclatures/Labels (Lecture)
• Microscope Maintenance & Cleaning (Lecture/Practical)
• Contrast Techniques: Phase Contrast & Dark Field, DIC & Polarisation, Epifluorescence basics (Lecture)
• Phase Contrast, DIC and Polarisation (Practical)
• Lenses, Open Optical Bench, NA & Swinging Apertometer (Practical)
• Flexible session; this may run subject to remaining time available and may include:
  • Epifluorescence microscopy (Practical)
  • Cameras, Image Capture, considerations for image analysis: (Pixels, Chip Type, Resolution, Signal to Noise, Dynamic Range & Scaling) (Lecture)